MONICA CULEA*, SILVIA NEAMŢU**, O. COZAR*
*“Babeş-Bolyai” University, 1, Kogălniceanu st., 400084 Cluj-Napoca, Romania
**INCDTIM, 65–103, Donath st., Cluj-Napoca, Romania
Abstract. The aim of this work was to study 15N-amino acid membrane transport by using isotope dilution GC/MS method in human erythrocyte in different conditions. The quantitation of glycine, as trifluoroacetyl butyl ester derivative was performed by isotope dilution technique, in the selected ion monitoring (SIM) mode. Washed cells were suspended in the incubation medium containing 15N-glycine in the range 1–10 mM and suspension was incubated in a rotary shaker at 37 C. The method was validated in the range 0–70 µg/ml and gave good linearity and reproducibility. Reproducibility gave C.V. lower than 14%. Our results proved that our technique provides feasible results for measuring amino acid transport. Increasing amino acid transport in the red cell related to temperature, time, amino acid concentration and electric field presence was determined.
Author’s e-mail: firstname.lastname@example.orgFull text: PDF