CRISTINA STAN, ANIȘOARA CÎMPEAN, DANA IORDĂCHESCU
Research Center for Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 93–95 Splaiul Independenţei, Bucharest, Romania
In psoriasis, disturbed differentiation, hyperproliferation, and inflammation are hallmarks that are clinically represented by scaling, plaque thickening, and erythema, respectively. The present study describes the response to UVA irradiation of cultured human keratinocytes isolated from lesional and simptomless skin biopsies obtained from a male patient in different stages of disease. The recurrence and remission phenomena are accompanied by keratinocyte alterations that suffer modification towards parental type – control keratinocytes from simptomless psoriatic skin (KCc) and keratinocytes from periphery of psoriatic plaque (KCps) respectively – without reaching the values of basis parameters: viability, proliferation and apoptosis. Keratinocyte UVA irradiation results in morphological changes, decrease of cell viability and proliferation and an increase in level of apoptosis. These parameters’ changes suggest the presence of genetic alterations in psoriasis. KCps appear to be more sensitive to UVA iradiation than KCc.
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