EARLY DETECTION OF LEAF SPOT DISEASE IN TINOSPORA CORDIFOLIA THROUGH CHLOROPHYLL FLUORESCENCE OJIP ANALYSIS

PREETPAL KAUR, JALPA DESAI, V. SONI#
Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, “Mohanlal Sukhadia” University, Udaipur – 313001, India

The aim of the present work was to study the impact of fungal pathogen Phoma putaminum Speg. on various photosynthetic parameters of tissues adjacent to the infection sites on leaves of Tinospora cordifolia which visually appear healthy. Changes in chlorophyll fluorescence data, specific and phenomenological fluxes, density of active reaction centers and maximal photochemical activity of PSII (Fv/Fm) indicate that toxins produced from P. putaminum impair photosynthesis before the appearance of visual symptoms of infection. The chlorophyll fluorescence OJIP analysis (The OJIP or JIP-test name comes from the specific points on the induction curve that is formed by the recorded chlorophyll fluorescence signals) revealed that changes of the photosynthetic apparatus at an early stage can be used as measurable markers for detection of leaf spot disease in T. cordifolia.
Key words: Tinospora cordifolia, leaf spot disease, chlorophyll florescence, JIP test.

Corresponding author’s e-mail: vineetsonijnu@gmail.com

Full text: PDF