DANIELA NEAGOŞ*, VALENTINA MITRAN**, GEORGETA CHIRACU*, ANIŞOARA CÎMPEAN**, R. COCOS*, DANA IORDĂCHESCU**
*University of Medicine and Pharmacy “Carol Davila”, Bucharest
**Research Center for Biochemistry and Molecular Biology, University of Bucharest, 93 Spl. Independenţei, Bucharest 5
Fibroblast culture systems are routinely used to investigate wound healing under a wide range of experimental conditions. To evaluate the effects of insulin, retinoic acid and hydrocortisone on wound healing we carried out the studies on a scraped cell monolayer and on a free floating fibroblast populated collagen lattice (FPCL) model. The extent of recovery of the scraped 2D surfaces and of the gel contraction was monitored by phase contrast microscopy, acquisition and analysis of images. Our data showed the positive effects of insulin on the wound healing behaviour of human dermal fibroblasts. Treatment with retinoic acid did not affect the healing process and hydrocortisone inhibited it.
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