FLORIN CIOBANU*, MIHAI RADU*,**, MIHAELA MOISESCU*, MARIUS SURLEAC*, LAURA BĂJENARU*, TUDOR SAVOPOL*, EUGENIA KOVÁCS*
*Department of Cellular Biophysics and Biotechnology, “Carol Davila” University of Medicine and Pharmaceutics, Bucharest, Romania
** Department of Health and Environmental Physics, „Horia Hulubei” National Institute for Physics and Nuclear Engineering, Măgurele, Romania
Abstract. In this work we intend to find optimal conditions (electric pulse amplitude and duration), in order to obtain high percentage of porated and viable cells by means of electroporation method. The experiments were done on B16F10 cell line. The poration yield and the viability of electroporated cells were evaluated following the ethidium bromide (EB) uptake in to the cells. Electric pulses (exponential decay) were used with the amplitude in the range of 0 – 6 kV/cm and pulse length of 100 µs and 200 µs. We obtained the highest percentage of viable porated cells for one or two pulses of 100 µs with the amplitude in the range of 1–2 kV/cm. The procedure described here offered a simple way to find optimal conditions of electroporation for malignant cells.
Key words: electroporation, electric field, cell culture.
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