G.M. NIȚULESCU, O.T. OLARU#, GEORGIANA NEDELCU, ANCA BUZESCU
Faculty of Pharmacy, „Carol Davila” University of Medicine and Pharmacy, 6, Traian Vuia st, Bucharest 020956, Romania
Using the aminopyrazole scaffold, N-[5-(4-bromophenyl)-1H-pyrazol-3-yl]carbamothioyl]benzamide was synthesized as a new anti-proliferative agent, hypothesized to target as a specific molecular substrate, several protein kinases. This compound (PRZ) showed anti-proliferative and cytotoxic effects in various human cancer cell lines. We assessed the effect of PRZ, along with the effects of cisplatin (CPT), methotrexate (MTX) and of their binary combinations with PRZ on Triticum aestivum root elongation test. We assessed phenomena such as antagonism or synergism, for a better understanding of the mechanisms underlying the anti-proliferative effect of PRZ. MTX and, in a lesser extent, PRZ inhibited cell growth and differentiation. More significant, their equimolar mixture had a more intense effect than MTX alone, but lower than the sum of the effects of each individual compound. The partially additive effect suggests a related mechanism of action for the two compounds. No significant effect was noticed for cisplatin alone, nor for its association with PRZ.
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