I.O. DOAGĂ, M. BOBOC
Department of Biophysics, “Carol Davila” University of Medicine and Pharmaceutics, București
Abstract. There are many clinical studies reporting the biostimulatory effects of He Ne laser on different biological processes like: wound healing, cell proliferation, tissue regeneration and hemostasis, but the exact mechanisms of these findings have never been revealed. Thus, we have considered a fundamental approach, trying to come up with some new biophysical evidences. In our experiments we have measured the human platelets membrane microviscosity before and after laser irradiation with different doses. The steady-state fluorescence anisotropy of membranes labeled with trimethylammonio-1,6-diphenyl-1,3,5-hexatriene (TMA-DPH) have been determined in different experimental protocols. The irradiation fluence was from 1.2 to 18 J∙cm–2, that covers the range used in the literature. No significant influences of He-Ne laser irradiation on membrane microviscosity have been observed. However, He-Ne laser increases the membrane fluidity of starved (near-exhausted) platelets.
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