VIDYARANI GEORGE*,***, M. JOHNSON*#, M. GLORY**
*Centre for Plant Biotechnology, Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli – 627002, Tamil Nadu, India
**Department of Botany, St. Mary’s College (Autonomous), Thuthukudi – 628001, Tamil Nadu, India
***Reg. No: 17212212142004), Affiliated to Manonmaniam Sundaranar University, Tirunelveli – 627 012, Tamil Nadu, India
In the present study, FT-IR analysis was used to study the functional groups present in the three different extracts of in vitro cultured gametophytes and sporophytes of Phlebodium aureum (L.) J. Smith. The powdered sample of each sample was loaded in FT-IR spectrophotometer (Shimadzu 8400S) in the range of 400 to 4000 cm–1. The transmission percentage was verified against the wave number. The peak values of FT-IR were recorded, and the functional groups were predicted using Aldrich and Sigma IR chart table. The analysis revealed the presence of different types of compounds such as alkene, sulfoxide, esters, aromatic compounds, isothiocyanate etc. The observed results showed the similarities and variation between the gametophytes and sporophytes of Phlebodium aureum. The outcome of the study showed the unique profiles for gametophytes and sporophytes, and these may be employed to identify the gametophytes and sporophytes of Phlebodium aureum.
Key words: Phlebodium aureum, gametophyte, sporophyte, functional groups
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