I.O. DOAGĂ*, E. RADU**, GYÖNGYVÉR KATONA***, TEOFILA SEREMET****, MARIA DUMITRESCU****, SÂNZIANA RADESI****, MIHAELA PISLEA****, JUDIT HORVÁTH*****, E. TANOS*****, L. KATONA****, EVA KATONA****
*Department of Biophysics, Dentistry Faculty, **Department of Molecular and Cellular Medicine, ***Department of Medical Biochemistry, and ****Department of Biophysics, Medical Faculty, “Carol Davila” University of Medicine and Pharmaceutics, Bucharest, Romania
*****LASEUROPA CO., Budapest, Hungary, www.softlaser.hu
We investigated the effects of low power 680 nm far-red and 830 nm near-infrared laser light on noninjured and energy/nutrient restricted human acute T leukemic Jurkat cells mitochondrial membrane state. Nutrient restriction engendered increase in mitochondrial membrane potential (m) of the cell subpopulation with highly polarized mitochondrial membrane (HIGH), and decrease in m of the subpopulation with low polarization mitochondrial membrane (LOW), changes induced by glucose deprivation with blockade of glycolysis being more substantial than those brought on by serum starvation. Glucose starvation also caused increase in the relative magnitude of the HIGH subpopulation, while serum starvation resulted in decrease in the relative magnitude of the HIGH subpopulation. Energy restriction induced by mild blockade of oxidative phosphorylation with low concentrations of cyanide caused increase in the relative magnitude of the HIGH subpopulation, while high concentration cyanide poisoning promoted mitochondrial membrane depolarization. Low level near infrared laser irradiation caused decrease in the HIGH subpopulation in control cell populations, partially reversed glucose starvation caused effects, while had no significant influence on mitochondrial membrane state in serum starved cells. Low level far-red laser irradiation induced increase in the relative magnitude of the HIGH subpopulation, and partially reversed mild and severe cyanide intoxication effects. Intermediate level cyanide intoxication caused increase in the relative magnitude of the HIGH subpopulation could also be enhanced by far-red laser radiation. In conclusion, our data show that soft laser irradiation significantly modulates the control and nutrient/energy restricted human T leukemia lymphoblasts mitochondrial membrane state in a dose and irradiation regime dependent manner.
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