IOANA AMĂRIUȚEI*, MARINELA FLOREA*#, IOANA CLEMENTINA CONSTANTINESCU*, MIHAELA ILIE**, CRINA-MARIA MONCIU*, CORINA-CRISTINA ARAMĂ*
* Department of Analytical Chemistry, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
**Department of Toxicology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
A new microemulsion electrokinetic chromatography (MEEKC) method has been developed for the determination of metformin, gliclazide and glibenclamide, using a microemulsion consisting of 3.3% SDS, 6.6% n-butanol, 0.8% n-heptane and 89.3% 20 mM borate buffer (pH 9.0) as running electrolyte, 23 kV applied voltage at 30 °C, with direct UV detection at 208 nm. The method was found to be linear between 30 and 300 µg/mL for each drug. Quantitative results were calculated in respect to peak area and in respect to peak area versus time ratio, the latter providing more accurate results for the highly lipophylic sulfonylurea drugs.
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