A.M.M. ATTIA*, GHADA M. NABIL* E.F. ABU-ATIAH**
*Biochemistry Department, National Research Centre, Cairo, Egypt
**Cairo Police Equestrian Department, Cairo, Egypt
Abstract. The fractions and concentrations of the inactive Hb pigments (such as sulfhemoglobin, SHb, methemoglobin, MetHb, carboxyhemoglobin, HbCO) and the active Hb (in the oxyhemoglobin form) as well as the total blood Hb concentration were determined in canine and bovine blood, using newly developed multi-component spectrophotometric methods. The results obtained by the cow’s method revealed values of MetHb% up to 4.607% and of HbCO% up to 4.814%, which are relatively higher than those values in canine blood (up to 1.385% and 3.0%, respectively). These high values of MetHb% and HbCO% in bovine blood may account for the observed decrease in the oxygen-carrying capacity of bovine blood Hb (HbO2%), when compared to canine blood. Furthermore, these methods has yielded values of the cow’s total blood Hb concentration up to 16.161 g dL–1 which are relatively lower than those values of dogs (up to 19.992 g dL–1). Moreover, these methods has yielded values of the cow’s blood HbO2 concentration up to 15.233 g dL–1 which are relatively lower than those values of dogs (up to 19.599 g dL–1). In conclusions, the results obtained by the methods suggested here for Hb-derivatives determinations showed significantly higher values of inactive Hbs % (MetHb % and HbCO %) concomitant with significant lower values of the active Hb (in the HbO2 form) in bovine blood, when compared to those values of canine blood. The results of these methods showed also significantly higher values of the total blood Hb-concentration and HbO2 concentration in dogs than cows.
Key words: multi-component spectrophotometric analysis, hemoglobin, sulfhemoglobin, methemoglobin, carboxyhemoglobin, oxyhemoglobin, cows, dogs.