A.M.M. ATTIA, GHADA M. NABIL, FATMA A. IBRAHIM, NAHED S. HASSAN
Biochemistry Department, National Research Centre, Cairo, Egypt
Abstract. The fractions and concentrations of the inactive Hb pigments (such as sulfhemoglobin, SHb, methemoglobin, MetHb, carboxyhemoglobin, HbCO) and the active Hb (in the HbO2 form) as well as the total blood Hb concentration were determined in mice and rat blood, using newly developed multi-component spectrophotometric methods. These methods have yielded values of MetHb% up to 3.514% and 2.914 in mice and rat blood, respectively. These methods have also yielded values of HbCO% up to 6.581% and 8.992% in mice and rat blood, respectively. The results revealed also values of SHb% up to 0.674% and 0.374% in mice and rat blood, respectively. The results revealed values of HbO2% up to 93.842% and 92.103% in mice and rat blood, respectively. Furthermore, these methods has yielded values of the mice’s total blood Hb concentration up to 15.994 g dL–1, which are relatively higher than those values of rats (up to 12.410 g dL–1). Moreover, these methods has yielded values of the mice’s blood HbO2 concentration up to 14.682 g dL–1, which are relatively higher than the values of rats (up to 11.398 g dL–1). In conclusions, the results obtained by the methods suggested here for Hb-derivatives determinations showed significantly higher values of HbCO% concomitant with significant lower values of the SHb% in rat blood, when compared to those values of mice blood. The results showed also insignificant differences in MetHb% and HbO2% between mice and rat blood. The results revealed also insignificant differences in total Hb-concentration and HbO2 concentration between mice and rats.
Key words: multi-component spectrophotometric analysis, hemoglobin, sulfhemoglobin, methemoglobin, carboxyhemoglobin, oxyhemoglobin, mice, rats.
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