EVA KATONA*, GYÖNGYVÉR KATONA**, I.O. DOAGA***, DIANA IONESCU*, R. MATEI*, JUDIT HORVÁTH****, E. TANOS****, L. KATONA*
*Department of Biophysics and **Department of Medical Biochemistry, Medical Faculty, ***Department of Biophysics, Dentistry Faculty, “Carol Davila” University of Medicine and Pharmaceutics, 8 Eroii Sanitari Blvd, sect. 5, 050474 Bucharest, Romania
****LASEUROPA CO., Budapest, Hungary, www.softlaser.hu
Abstract. The aim of the present study was to supply new data concerning the cellular effects of low power long wavelength laser irradiation. Steady state fluorescence intensity measurements with the use of appropriate fluorophores allowed disclosure of changes induced by the 680 nm red and the 830 nm infrared laser radiation in the polar headgroup region lipid order parameter of plasma membranes as well as in the intracellular calcium levels of human platelets and peripheral blood lymphocytes. Comparison of the effects seen in freshly separated and in metabolically impaired cells revealed a sensitive modulation of laser irradiation effects by the actual metabolic state of the cells.
Key words: AlGaInP/GaAs laser, metabolic impairment, fluorescence anisotropy, TMA-DPH, cell calcium, Fura2 AM.
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