TEOFILA SEREMET*, MARIA DUMITRESCU*, SANZIANA RADESI*, GYÖNGYVÉR KATONA**, I.O. DOAGA****, E. RADU***, JUDIT HORVÁTH*****, E. TANOS*****, L. KATONA*, EVA KATONA*
*Department of Biophysics, **Department of Medical Biochemistry and ***Department of Molecular and Cellular Medicine, Medical Faculty, ****Department of Biophysics, Dentistry Faculty, “Carol Davila” University of Medicine and Pharmaceutics, Bucharest, Romania; e mail: email@example.com
*****LASEUROPA CO., Budapest, Hungary, http://www.softlaser.hu
Abstract. In this report we investigated the effects of low power 680 nm far-red and 830 nm near-infrared laser irradiation on quercetin-induced cell growth inhibition and apoptosis induction. High micromolar concentrations (100µM) of quercetin decreased cell viability and prevented the proliferation of human T leukemic lymphoblasts (Jurkat) in a time-dependent manner. Flow cytometry data attested cell cycle arrest in the S phase and apoptosis induction. Low dose (~1 µJ/cell) laser irradiation significantly modulated the quercetin effects seen in human leukemic cells. Near-infrared laser light partially reversed apoptosis induction and promoted cell cycle progression in the G2 phase, in a dose-dependent manner, while far-red laser light potentiated quercetin-toxicity.
Key words: AlGaInP/GaAs laser, quercetin-induced stress, viability, proliferation rate, cell cycle progression, apoptosis.
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