BOGDANA VÎRGOLICI*, MARIA MOHORA*, LAURA GĂMAN*, DANIELA LIXANDRU*, B. MANOLESCU*, ANCA COMAN**, IRINA STOIAN*
*Department of Biochemistry, “Carol Davila” University of Medicine and Pharmaceutics, Bucharest, Romania
**”N. Paulescu” Institute of Diabetes, Nutrition and Metabolic Diseases, Bucharest, Romania
Abstract. Oxidative stress and carbonylic stress are associated with diabetes mellitus. It is not known if they are cause or effect in this illness. Also, chronic low-grade inflammation is involved in the pathogenesis of type 2 diabetes. The aim of this study is to assess the relationship between inflammation markers levels and blood redox status in diabetic foot patients.
Forty newly hospitalized diabetic foot patients, aged between 45–75, were enrolled. Considering C-reactive protein (CRP) values, patients were divided in two groups: group 1 with CRP 0,1–1 mg/dL and group 2 with CRP values above 1 mg/dL. Blood samples were also collected from twenty healthy controls, age and sex-matched. Spectrophotometric methods were applied for oxidative stress markers.
Plasma concentrations for dROM (determinable reactive oxygen metabolites), CRP, ceruloplasmin, dicarbonyls, uric acid and blood glutathione were higher in diabetic patients vs. controls. Plasma total antioxidant capacity was not significantly modified. Comparing the diabetic patients, plasma concentrations of dROM, ceruloplasmin, uric acid and glycated hemoglobin were significantly increased in group 2 vs. group 1. Blood glutathione, plasma dicarbonyls and total antioxidant capacity values were similar in both groups. Correlations between plasma dROM and ceruloplasmin and also between uric acid and glycated hemoglobin were calculated for both groups.
This study suggests that the link between obesity and diabetes mellitus may be represented by inflammation and oxidative stress. Modulating these processes using therapeutic methods and of course, weight control may reduce the incidence of diabetic complications.
Key words: inflammation, oxidative stress, obesity, diabetes mellitus.
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