DANIELA NEAGOŞ, VALENTINA MITRAN, GEORGETA CHIRACU, RALUCA CIUBĂR, CRISTINA IANCU, CRISTINA STAN, ANIŞOARA CÎMPEAN, DANA IORDĂCHESCU
*“Carol Davila” University of Medicine and Pharmaceutics, 8 Eroii Sanitari Bvd, 050474, Bucharest, Romania
**Research Center for Biochemistry and Molecular Biology, University of Bucharest, 93, Splaiul Independenţei, Bucharest, Romania
Abstract. Wound healing is a highly regulated process that involves reepithelization, granulation tissue formation and contraction. The aim of this paper is to study on a free floating fibroblast populated collagen lattice (FPCL) model the release of IL-1α and IL-1β in the medium and the pattern of the expressed matrix metalloproteinases MMP-2 and MMP-9 as a result of mechanical, oxidative, irritant and gamma irradiation induced lesions. Pro-inflammatory cytokines are clearly involved in the regulation of the wound healing. We studied the level of the extracellular IL-1α and IL-1β and found out a higher expression of the IL-1β in the first day of culture and a decrease of both IL-1 after 14 days of culture. Different phases of the wound healing involve some matrix metalloproteinase members and their tissue inhibitors. In our study, the zymographic analysis demonstrated the presence of MMP-2 and MMP-9 in the medium of fibroblasts grown in the three dimensional collagen network. The levels of MMP-2 and MMP-9 were gradually increasing in the first 5 days of culture, probably as a result of the action of IL-1β on the process of MMP activation. Our results recommend the three dimensional model of fibroblast culture in collagen gels as a useful model of in vitro study of wound healing.
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