İ. DOROBANŢU, LİVİA HARANGUŞ, M. RADU
“Horia Hulubei” National Institute for Physics and Nuclear Engineering (IFIN-HH), 407, Atomiştilor st., Măgurele, 077125, Romania
Abstract. A procedure of synthesis of the enzymatic marker 3,6-dichloro-2-methoxybenzoic-alcaline phosphatase is described. This bioconjugate is used as reagent in ELISA technique to monitor this pesticide from environmental samples. According the procedure, 3,6-dichloro-2-methoxybenzoic acid is activated with N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in anhydrous medium of dimethylformamide and then coupled to alkaline phosphatase. The enzymatic activity and the evaluation of the affinity against homologue antipesticide antibody are also described.
Key words: immunoassay, dicamba, affinity, antipesticide, antibody.
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