SILVIA JULIET IRUTHAYAMANI*, M. JOHNSON**#
*Reg. No. 19211282262014, Affiliated to Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India 627012
**Centre for Plant Biotechnology, Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tamil Nadu, India 627002
In the present study, an attempt is made to reveal the functional groups and metabolites of gametophyte and sporophyte of two pteridophytes Anemia schimperiana C. Presl subsp. wightiana (Gardner) Fraser-Jenk. and Cyathea gigantea (Wall ex Hook.) Holtt using UV-Vis and FTIR analysis. For UV-Vis analysis, the ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were centrifuged at 3000 rpm for 5 min and filtered using Whatman No. 1 filter paper. The ethanolic extracts were scanned and recorded using Shimadzu UV-Vis spectrophotometer at the absorbance of 200 to 1100 nm. For FTIR spectrometry about 1.0 mg of gametophyte and sporophyte ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were separately made into very thin discs with potassium bromide and the pellets are measured in an automatic recording IR Spectrophotometer in the range of 400–4000 cm–1. Based on the observed peak values, the existence of alkaloids, phenolics, tannin and carotenoid existence were confirmed. Terpenoids, flavonoid and chlorophyll existence were also validated in different peak values of the ethanolic extracts of gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The FTIR analysis results showed various functional groups viz., aromatic compounds, alkene, fluoro compounds, anhydride, aliphatic ether, alcohol, secondary aminepresence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The gametophyte and sporophyte stages expressed the existence of different functional groups and displayed the variations and similarities between the studied stages. The spectroscopic results confirmed the secondary metabolites existence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea ethanolic extracts. Further chromatographic and separation studies may bring out stage-specific bioactive principles from the gametophytes and sporophytes of studied ferns.
Key words: Anemia schimeriana subsp. wightiana, Cyathea gigantea, functional group, gametophyte, sporophyte