Z. TRUȚĂ, SILVIA NEAMȚU, V.V. MORARIU
National Institute for R & D of Isotopic and Molecular Technologies, P.O.Box 700, Cluj-Napoca 5, 400293, Romania
The effect of zero magnetic field environments (ZMF) on human semen was investigated in connection with the main natural fertilization parameters: viability and motility of spermatozoa cells. Semen samples were exposed for 30 hours in ZMF created in a pair of Helmoltz coils and in geomagnetic field (GMF) as control at 20°C. Cells population aging was estimated by the decrease of rapid progressive cells viability. An imaging analysis method was used to characterize cells velocity. We found that in ZMF the decrease of cells viability was delayed with about five hours compared to GMF. Rapid progressive spermatozoa cells velocity naturally slows down at 200C after four hours; cells become slow progressive or immobile after 20 hours. A significantly enhancement of cells motility was recorded in the first period of ZMF exposure. In this conditions cells velocity is maintained around the initial values even after 20 hours. We concluded that zero magnetic field slow down the aging of spermatozoa cells population, and stimulate cells velocity in vitro conditions.
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