FTIR SPECTROSCOPIC STUDIES ON CLEOME GYNANDRA - COMPARATIVE ANALYSIS OF FUNCTIONAL GROUP BEFORE AND AFTER EXTRACTION

DEEPASHREE. C. LINGEGOWDA*, J. KOMAL KUMAR**, A.G. DEVI PRASAD** MAHSA ZAREI***, SHUBHA GOPAL*

*Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India;
**Department of Studies in Environmental Science, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
***Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India

FTIR spectra of Cleome gynandra leaf powder before and after extraction with methanol were recorded. The frequency range and intensities were obtained from absorption spectra. Compression of the constituents present before and after extraction was made. The results have shown that the biomolecules were rich in the sample before extraction as compared to after extraction. The result indicates that the sample before extraction was rich in certain constituents than after extraction. This analysis showed the presence of hydroxyl compounds, carbonyl compounds, amines, halogens compounds in both the sample. The analysis also revealed the presence of different types of biomolecules in both extracts. The sample prior to extraction contains aromatic compounds, halogen compounds, amides, where as sample after extraction showed the presence of functional groups like carboxyl acid, alkyl amine, alkyl halides.

Corresponding author’s e-mail: sguom@yahoo.in

Full text: PDF