Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt
Abstract. It is well known, that serum components destabilize liposomal membranes. Therefore, most in vitro transfection protocols avoid serum, which make the extrapolation to in vivo situations difficult. In this study, we investigated the stability of different anionic liposomal formulations including AVEs (Artificial Viral Envelopes) in 100% fetal calf serum (FCS) by measuring the efflux of the entrapped carboxyfluorescein (CF). The results suggest that lysis of AVE liposomes in serum can be decreased by incorporating sphingomyelin (SM) in the liposomal membrane and using highly lipid doses.
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